Gargle Nucleic Acide Gukuramo Reagents
IHame RY'INGENZI
Ibicuruzwa bigizwe ahanini na gargle, ivanga cyane superparamagnetic nanospheres ivanze, hamwe na lysis reagents idasanzwe.Isaro idasanzwe ya magnetiki yashyizwemo ifitanye isano nibintu bigize umubiri (harimo virusi yubusa na selile zanduye virusi).Iyo uhuye na lysis igisubizo, selile idafite ionic / nucleus-membrane-yamenagura surfactants hamwe na protease inhibitor mugisubizo gishobora kubuza ibikorwa bya enzyme ya ADN / RNA, kandi igahagarika aside nucleique.Ibintu byose bya acide nucleic mubice bigize umubiri wa gargle birekurwa neza mumuti wa lysis, bigahita bibona aside nucleic.Ingero za gargle ukoresheje iki gikoresho ntizisaba kweza aside nucleic, zishobora gukoreshwa muburyo bwo gutahura aside nucleic aside.
Ibice byingenzi bigize reagent
Ibigize bigaragara mu mbonerahamwe ya 1.
Imbonerahamwe 1 Ibigize no gupakira muri Kit
| Izina ryibigize | Ibice nyamukuru | Ingano (1) | Ingano (10) | Ingano (30) | Ingano (50) |
| 1. Gargle A. | NaCl | 8mL / tube | 8mL / tube * imiyoboro 10 | 8mL / tube * igituba 30 | 8mL / tube * imiyoboro 50 |
| 2. Umukoresha wa Gargle | PP | Igice 1 | 10 pc | 30 pc | 50 pc |
| 3. Igisubizo gikungahaye B. | Amasaro ya rukuruzi | 2mL / tube | 2mL / tube * imiyoboro 10 | 2mL / tube * igituba 30 | 2mL / tube * imiyoboro 50 |
| 4 Lysis Buffer C. | Kurinda K. | 0.2mL / igice | 0.2mL / igice * 10 pc | 0.2mL / igice * 30 pc | 0.2mL / igice * 50 pc |
| 5. Umuyoboro wa rukuruzi | Magnet | Igice 1 | 10 pc | 30 pc | 50 pc |
Ibigize bifite agaciro mumezi 12.
Ibice bisabwa mugukuramo aside nucleic, ariko ntibishyizwe mubikoresho:
1. Ibikoreshwa: 1.5ml umuyoboro wa EP;
2. Ibikoresho: Kwiyuhagira amazi (cyangwa kwiyuhagira ibyuma), imiyoboro, na centrifuge.
Amakuru y'ibanze
Icyitegererezo gisabwa:
1. Bikoreshwa mugukuramo aside nucleic no gutunganyiriza ingero za gargle.
2. Icyitegererezo cya gargle kizongerwaho igisubizo gikungahaye B mugihe cyo gukusanya.Amasaro ya magnetiki yagaruwe agomba kwimurirwa muri lysis buffer C ako kanya.Ingero zongewe kuri lysis buffer C irashobora kubikwa mubushyuhe bwicyumba.
Ibipimo byo gupakira: Igice 1 / agasanduku, 10 pc / agasanduku, 30 pc / agasanduku, na 50 pc / agasanduku.
Imiterere yo kubika: Igisubizo gikungahaye B na lysis igisubizo C bigomba kubikwa amezi 12 kuri 2-8 ℃, nibindi bice bishobora kubikwa amezi 12 munsi yubushyuhe bwicyumba;Igikoresho gishobora gutwarwa no kubikwa by'agateganyo munsi yubushyuhe bwibidukikije, ibyo ntibishobora kurenza iminsi 5.
Igihe cyemewe: Amezi 12
Icyemezo cyibikoresho byubuvuzi Icyemezo No.HJXB No 20220086.
Itariki yo kwemezwa no gusubiramo Amabwiriza:
Itariki yemejwe: 26 Ukwakira 2022
