TAGMe ADN Methylation Detection Kits (qPCR) ya Kanseri ya Endometrale
IBIKURIKIRA
Icyitonderwa
Byemejwe n’amavuriro arenga 800 mu bushakashatsi bubiri-buhumye-bushakashatsi, ibicuruzwa bifite umwihariko wa 82.81% hamwe na sensibilité ya 80.65%.
Byoroshye
Tekinoroji yambere ya Me-qPCR methylation detection irashobora kurangizwa muntambwe imwe mugihe cyamasaha 3 nta bisulfite ihinduka.
Kera
Kugaragara kurwego rwibanze.
Kwikora
Bikurikizwa hamwe na brush cervical brush na Pap smear sample.
UKORESHEJWE
Iki gikoresho gikoreshwa muri vitro yujuje ubuziranenge bwa hypermethylation ya gene PCDHGB7 ingero zidasanzwe.Igisubizo cyiza cyerekana ibyago byinshi byo kwandura indwara ziterwa na endometrale na kanseri, bisaba ko hasuzumwa amateka ya endoprium.Ibinyuranye na byo, ibisubizo bibi by'ibizamini byerekana ko ibyago byo kwandura indwara ya endometrale na kanseri ari bike, ariko ibyago ntibishobora kuvaho rwose.Isuzuma ryanyuma rigomba gushingira kubisubizo bya histopathologique ibisubizo bya endometrium.PCDHGB7 ni umwe mu bagize umuryango wa protocadherin γ gene cluster.Habonetse Protocadherin igenga imikorere y’ibinyabuzima nko gukwirakwiza ingirabuzimafatizo, ingirabuzimafatizo, apoptose, gutera, kwimuka na autofagy ya selile yibibyimba binyuze mu nzira zitandukanye zerekana ibimenyetso, kandi gucecekesha gene kwayo guterwa na hypermethylation y'akarere ka porotokoro bifitanye isano rya bugufi no kubaho no gutera imbere ya kanseri nyinshi.Byavuzwe ko hypermethylation ya PCDHGB7 ifitanye isano n’ibibyimba bitandukanye, nka lymphoma itari Hodgkin, kanseri y'ibere, kanseri y'inkondo y'umura, kanseri y'inda na kanseri y'uruhago.
IHame RY'INGENZI
Iki gikoresho kirimo gukuramo aside nucleic reagent na reagent ya PCR.Acide nucleique ikurwa muburyo bwa magnetiki-isaro.Iki gikoresho gishingiye ku ihame ryuburyo bwa PCR bwa fluorescence, ukoresheje methylation yihariye-nyayo-nyayo ya PCR kugirango isesengure ADN yerekana icyitegererezo, kandi icyarimwe igaragaze imbuga za CpG za gen PCDHGB7 hamwe nikimenyetso cyo kugenzura ubuziranenge imbere ibice bya G1 na G2.Urwego rwa methylation ya PCDHGB7 murugero, cyangwa agaciro ka Me, rubarwa ukurikije PCDHGB7 gene methylated ADN amplification ya Ct agaciro na Ct agaciro kerekana.PCDHGB7 gene hypermethylation nziza cyangwa mbi igenwa ukurikije agaciro ka Me.
Ibisabwa
Kwerekana hakiri kare
Abantu bafite ubuzima bwiza
Isuzuma rya Kanseri
Amatsinda afite ibyago byinshi (abantu bafite amaraso adasanzwe mu gitsina nyuma yo gucura, kubyimba kwa endometrale, nibindi)
Gukurikirana
Umubare w'abaturage
Ubusobanuro bwa Clinical
Kwipimisha hakiri kare kubaturage bazima:Kanseri ya endometrale hamwe nibisebe bibanziriza bishobora gusuzumwa neza;
Isuzuma ry’ingaruka ku baturage bafite ibyago byinshi:Isuzumabumenyi rishobora gukorwa kubantu bafite amaraso adasanzwe mu gitsina no kubyimba kwa endometrale nyuma yo gucura kugirango bafashe mugupima indwara;
Ikurikiranwa ryabaturage risubirwamo:Igenzura ry’abaturage nyuma yo kubagwa rishobora gukorwa kugirango hirindwe gutinda kwatewe no kugaruka.
Icyegeranyo cy'icyitegererezo
Uburyo bwo gutoranya: Shira igitereko cyimyanya ndangagitsina kuri os cervical os, kwoza buhoro buhoro umwanda winkondo y'umura hanyuma uzenguruke inshuro 4-5 isaha yisaha, ukureho buhoro buhoro umwanda winkondo y'umura, ubishyire mubisubizo byo kubika selile, hanyuma ubishyireho ikimenyetso kugirango ukore ibizamini bikurikira.
Kubungabunga ingero:Ingero zirashobora kubikwa mubushyuhe bwicyumba mugihe cyiminsi 14, kuri 2-8 ℃ mugihe cyamezi 2, na -20 ± 5 ℃ mugihe cyamezi 24.
Igikorwa cyo gutahura: Amasaha 3 (Hatabayeho inzira y'intoki)
TAGMe ADN Methylation Detection Kits (qPCR) ya Kanseri ya Endometrale
| Gusaba ivuriro | Kwipimisha kwa Clinical infashanyo ya kanseri ya endometrale |
| Gene | PCDHGB7 |
| Ubwoko bw'icyitegererezo | Inkondo y'umura y'abagore |
| Uburyo bwo kugerageza | Fluorescence ingano ya tekinoroji ya PCR |
| Ingero zikoreshwa | ABI7500 |
| Gupakira ibisobanuro | Ibizamini 48 |
| Ububiko | Kit A igomba kubikwa kuri 2-30 ℃ Kit B igomba kubikwa kuri -20 ± 5 ℃ Byemewe kugeza kumezi 12. |
Ibyerekeye Twebwe
Epiprobe ifite ibikorwa remezo byuzuye: Ikigo cy’ibikorwa bya GMP gifite ubuso bwa metero kare 2200, kandi kigakomeza gahunda yo gucunga neza ISO13485, yujuje ibyangombwa bisabwa by’ubwoko bwose bw’ibizamini bya geneti;laboratoire yubuvuzi ifite ubuso bwa metero kare 5400 kandi ifite ubushobozi bwo gukora ubucuruzi bwa methylation ya kanseri nka laboratoire yubuvuzi yemewe.Byongeye kandi, dufite ibicuruzwa bitatu twabonye icyemezo cya CE, gikubiyemo kanseri y'inkondo y'umura, kanseri ya endometrale na kanseri ya urothelia.
Ikoranabuhanga rya Epiprobe ryerekana kanseri ya kanseri irashobora gukoreshwa mugupima kanseri hakiri kare, gusuzuma indwara zifasha, gusuzuma mbere na nyuma yo kubagwa, kugenzura recrudescence, ikora inzira yose yo gusuzuma no kuvura kanseri, itanga ibisubizo byiza kubaganga n'abarwayi.
