TAGMe ADN Methylation Yerekana Ibikoresho (qPCR) kuri Kanseri ya Urothelia
IBIKURIKIRA
Icyitonderwa
Byemejwe hejuru yubuvuzi burenga 3500 mubushakashatsi bubiri-buhumye-bushakashatsi, ibicuruzwa bifite umwihariko wa 92.7% hamwe na sensibilité ya 82.1%.
Byoroshye
Tekinoroji yambere ya Me-qPCR methylation detection irashobora kurangizwa muntambwe imwe mugihe cyamasaha 3 nta bisulfite ihinduka.
Kudatera
30 mL yonyine y'icyitegererezo cy'inkari zirasabwa kumenya ubwoko 3 bwa kanseri, harimo kanseri y'impyiko, kanseri y'inkari, kanseri y'uruhago icyarimwe.
Ibisabwa
Gusuzuma ubufasha: Abarwayi bafite kanseri ya urothelia barashobora kwisuzumisha muburyo budatera kugirango bafashe mugupima indwara.
Kubaga / gusuzuma imiti ya chimiotherapie: Suzuma akamaro ko kubaga / chimiotherapie kugirango ufashe kunoza ivuriro ingaruka zo kuvura.
Gukurikirana umubare wabaturage nyuma yo kugaruka:Abarwayi barwaye kanseri ya urothelia barashobora gukurikiranwa kugirango bagaruke muburyo budatera, muburyo budatera, buteza imbere abarwayi.
Icyegeranyo cy'icyitegererezo
Uburyo bwo gutoranya: Uburyo bwo gutoranya: Kusanya icyitegererezo cy'inkari (inkari zo mu gitondo cyangwa inkari zidasanzwe), ongeramo igisubizo cyo kubika inkari hanyuma uvange neza, ubibike ku bushyuhe bw'icyumba hanyuma ubishyireho ikimenyetso gikurikira.
Kubungabunga ingero: Ingero zirashobora kubikwa mubushyuhe bwicyumba mugihe cyiminsi 14, kuri 2-8 ℃ mugihe cyamezi 2, na -20 ± 5 ℃ mugihe cyamezi 24.
Igikorwa cyo gutahura: Amasaha 3 (Hatabayeho inzira y'intoki)
Ibikoresho bya ADN Methylation (QPCR) kuri Kanseri ya Urothelia
| Gusaba ivuriro | Kwipimisha kwa Clinical infashanyo ya urothelial cnacer;kubaga / gusuzuma imiti ya chimiotheray;gukurikirana nyuma yo gukurikiranwa |
| Gene | UC |
| Ubwoko bw'icyitegererezo | Inkari zifata ingirabuzimafatizo (inkari) |
| Uburyo bwo kugerageza | Fluorescence ingano ya tekinoroji ya PCR |
| Ingero zikoreshwa | ABI7500 |
| Gupakira ibisobanuro | Ibizamini 48 |
| Ububiko | Kit A igomba kubikwa kuri 2-30 ℃Kit B igomba kubikwa kuri -20 ± 5 ℃ Byemewe kugeza kumezi 12. |
