TAGMe ADN Methylation Yerekana Ibikoresho (qPCR) kuri Kanseri ya Urothelia
IBIKURIKIRA
Icyitonderwa
Byemejwe hejuru yubuvuzi burenga 3500 mubushakashatsi bubiri-buhumye-bushakashatsi, ibicuruzwa bifite umwihariko wa 92.7% hamwe na sensibilité ya 82.1%.
Byoroshye
Tekinoroji yambere ya Me-qPCR methylation detection irashobora kurangizwa muntambwe imwe mugihe cyamasaha 3 nta bisulfite ihinduka.
Kudatera
30 mL yonyine y'icyitegererezo cy'inkari zirasabwa kumenya ubwoko 3 bwa kanseri, harimo kanseri y'impyiko, kanseri y'inkari, kanseri y'uruhago icyarimwe.
Ibisabwa
Gusuzuma Ubufasha
Abaturage barwaye hematuria itababara / bakekwaho kuba bafite urothelia (kanseri yinkari / kanseri yimpyiko)
Isuzuma rya Kanseri
Kubaga / chimiotherapie-isaba abaturage bafite kanseri ya urothelia;
Gukurikirana
Abaturage nyuma yo kubagwa hamwe na kanseri ya urothelia
UKORESHEJWE
Iki gikoresho gikoreshwa muri vitro yujuje ubuziranenge bwa hypermethylation ya gen Urothelial Carcinoma (UC) muri urothelial.Igisubizo cyiza cyerekana ibyago byiyongera bya UC, bisaba ko hasuzumwa cystoskopi na / cyangwa isuzuma rya histopathologique.Ibinyuranye, ibisubizo bibi byikizamini byerekana ko ibyago bya UC ari bike, ariko ibyago ntibishobora kuvaho rwose.Isuzuma ryanyuma rigomba gushingira kuri cystoscope na / cyangwa ibisubizo bya histopathologique.
IHame RY'INGENZI
Iki gikoresho kirimo gukuramo aside nucleic reagent na reagent ya PCR.Acide nucleique ikurwa muburyo bwa magnetiki-isaro.Iki gikoresho gishingiye ku ihame ryuburyo bwa PCR bwa fluorescence PCR, ukoresheje methylation yihariye-nyayo-nyayo ya PCR kugirango isesengure ADN yerekana inyandikorugero, kandi icyarimwe igaragaze imbuga za CpG za UC gene hamwe nikimenyetso cyo kugenzura ubuziranenge imbere yimbere ya G1 na G2.Urwego rwa methylation ya UC gene, yitwa Me agaciro, ibarwa ukurikije UC gene methylated ADN amplification ya Ct agaciro na Ct agaciro kavuzwe.UC gene hypermethylation nziza cyangwa mibi igenwa ukurikije agaciro ka Me.
Ibikoresho bya ADN Methylation (QPCR) kuri Kanseri ya Urothelia
| Gusaba ivuriro | Kwipimisha kwa Clinical infashanyo ya urothelial cnacer;kubaga / gusuzuma imiti ya chimiotheray;gukurikirana nyuma yo gukurikiranwa |
| Gene | UC |
| Ubwoko bw'icyitegererezo | Inkari zifata ingirabuzimafatizo (inkari) |
| Uburyo bwo kugerageza | Fluorescence ingano ya tekinoroji ya PCR |
| Ingero zikoreshwa | ABI7500 |
| Gupakira ibisobanuro | Ibizamini 48 |
| Ububiko | Kit A igomba kubikwa kuri 2-30 ℃ Kit B igomba kubikwa kuri -20 ± 5 ℃ Byemewe kugeza kumezi 12. |
