Gukuramo Acide Nucleic (A01)
Ihame ryo gutahura
Nyuma yo kurekura ADN genomic mugabanye selile hamwe na lysis buffer, isaro ya magneti irashobora guhitamo guhuza ADN ya genomic muri sample.Umubare muto wumwanda winjizwa nisaro ya magneti urashobora gukurwaho na bffer yo gukaraba.Muri TE, isaro ya magnetiki irashobora kurekura ADN imipaka, ikabona ADN yo mu rwego rwo hejuru.Ubu buryo buroroshye kandi bwihuse kandi ADN yakuweho ni nziza, ishobora kuzuza ibisabwa kugirango hamenyekane methylation ya ADN.Hagati aho, ibikoresho byo gukuramo bishingiye ku masaro ya magnetiki birashobora guhuzwa no gukuramo aside nucleic aside yikora, byujuje imirimo myinshi yo gukuramo aside nucleic.
Ibice byingenzi bigize reagent
Ibigize bigaragara mu mbonerahamwe ya 1:
Imbonerahamwe 1 Ibigize reagent hamwe no gupakira
| Izina ryibigize | Ibice nyamukuru | Ingano (48) | Ingano (200) |
| 1. Umuti wa Lysis | Guanidine hydrochloride, Tris | 11 mL / icupa | 44 mL / icupa |
| 2. Gusukura ibisubizo A. | NaCl, Tris | 11 mL / icupa | 44 mL / icupa |
| 3. Gusukura ibisubizo B. | NaCl, Tris | 13 mL / icupa | 26.5mL / icupa * 2 |
| 4. Umuhanga | Tris, EDTA | 12 mL / icupa | 44 mL / icupa |
| 5. Protease K igisubizo | Kurinda K. | 1.1mL / igice | 4.4mL / igice |
| 6. Guhagarika amasaro ya rukuruzi 1 | Amasaro ya rukuruzi | 1.1mL / igice | 4.4mL / igice |
| 7. Amabwiriza yo gukuramo aside nucleic reagents |
| Kopi 1 | Kopi 1 |
Ibice bisabwa mugukuramo aside nucleic, ariko ntibishyizwe mubikoresho:
1. Reagent: Ethanol ya Anhydrous, isopropanol na PBS;
2. Ibikoreshwa: umuyoboro wa 50ml centrifuge na 1.5ml ya EP;
3. Ibikoresho: Isafuriya yo kogeramo amazi, pipettor, ububiko bwa magnetiki, centrifuge, isahani yimbitse 96 (byikora), ibikoresho byo gukuramo aside nucleic byikora (byikora).
Amakuru y'ibanze
Icyitegererezo gisabwa
1. Kwanduza umusaraba hagati yintangarugero bigomba kwirindwa mugukusanya no kubika.
2.Gushakisha bizarangizwa munsi yiminsi 7 yo kubika ubushyuhe bwibidukikije nyuma yo gukusanya icyitegererezo cyinkondo y'umura (kidakosowe).Kumenya bizarangira munsi yiminsi 30 yo kubika ubushyuhe bwibidukikije nyuma yo gukusanya inkari;Imenyekanisha rigomba kurangira mugihe cyo gukusanya ingirabuzimafatizo z'umuco.
Ibisobanuro bya parikingi:200 pc / agasanduku, 48 pc / agasanduku.
Imiterere yo kubika:2-30 ℃
Igihe cyemewe:Amezi 12
Igikoresho gikoreshwa:Tianlong NP968-C igikoresho cyo gukuramo aside nucleic, Tiangen TGuide S96 igikoresho cyo gukuramo aside nucleic, GENE DIAN EB-1000 igikoresho cyo gukuramo aside nucleic.
Icyemezo cyibikoresho byubuvuzi Icyemezo No. HJXB No 20210099.
Itariki yo kwemeza no guhindura amabwiriza:
Itariki yo kwemezwa: 18 Ugushyingo 2021
