Gukuramo Acide Nucleic (A02)
Ihame ryo gutahura
Nyuma yo kurekura ADN genomic mugabanye selile hamwe na lysis buffer, isaro ya magneti irashobora guhitamo guhuza ADN ya genomic muri sample.Umubare muto wumwanda winjizwa nisaro ya magneti urashobora gukurwaho na bffer yo gukaraba.Muri TE, isaro ya magnetiki irashobora kurekura ADN imipaka, ikabona ADN yo mu rwego rwo hejuru.Ubu buryo buroroshye kandi bwihuse kandi ADN yakuweho ni nziza, ishobora kuzuza ibisabwa kugirango hamenyekane methylation ya ADN.Hagati aho, ibikoresho byo gukuramo bishingiye ku masaro ya magnetiki birashobora guhuzwa no gukuramo aside nucleic aside yikora, byujuje imirimo myinshi yo gukuramo aside nucleic.
Ibice byingenzi bigize reagent
Ibigize bigaragara mu mbonerahamwe ya 1:
Imbonerahamwe 1 Ibigize reagent hamwe no gupakira
| Izina ryibigize | Ibice nyamukuru | Ingano (48) | Ingano (200) |
| 1. Ifunguro ryibiryo A. | Tris, SDS | 15.8 mL / icupa | 66mL / icupa |
| 2. Lysis Buffer L. | Guanidinium Isothiocyanate, Tris | 15.8 mL / icupa | 66mL / icupa |
| 3. Karaba Buffer A. | NaCl, Tris | 11 mL / icupa | 44 mL / icupa |
| 4. Karaba Buffer B. | NaCl, Tris | 13 mL / icupa | 26.5mL / icupa * 2 |
| 5. TE | Tris, EDTA | 12 mL / icupa | 44 mL / icupa |
| 6. Protease K igisubizo | Kurinda K. | 1.1mL / igice | 4.4mL / igice |
| 7. Guhagarika amasaro ya magneti 2 | Amasaro ya rukuruzi | 0.5mL / igice | 2.2mL / igice |
| 8. Amabwiriza yo gukuramo aside nucleic reagents | / | Kopi 1 | Kopi 1 |
Ibice bisabwa mugukuramo aside nucleic, ariko ntibishyizwe mubikoresho:
1. Reagent: Ethanol ya Anhydrous, isopropanol, na PBS;
2. Ibikoreshwa: umuyoboro wa 50mL centrifuge na 1.5mL EP;
3. Ibikoresho: Kwiyuhagira amazi, pipeti, ububiko bwa magnetiki, centrifuge, isahani 96-nziza (byikora), ibikoresho byo gukuramo aside nucleic byikora (byikora).
Amakuru y'ibanze
Icyitegererezo gisabwa:
1.Kumenyekanisha bigomba kurangizwa munsi yiminsi 7 yo kubika ubushyuhe bwibidukikije nyuma yo gukusanya icyitegererezo cyinkondo y'umura (kidakosowe).
2.Kumenyekanisha bigomba kurangira munsi yiminsi 30 yabitswe nubushyuhe bwibidukikije nyuma yo gukusanya icyitegererezo cyinkondo y'umura (cyagenwe) ..
3.Kumenyekanisha bigomba kurangira munsi yiminsi 30 yo kubika ubushyuhe bwibidukikije nyuma yo gukusanya inkari;Imenyekanisha rigomba kurangira mugihe cyo gukusanya ingirabuzimafatizo z'umuco.
Ibisobanuro bya parikingi:200 pc / agasanduku, 48 pc / agasanduku.
Imiterere yo kubika:2-30 ℃
Igihe cyemewe:Amezi 12
Igikoresho gikoreshwa:Tianlong NP968-C igikoresho cyo gukuramo aside nucleic, Tiangen TGuide S96 igikoresho cyo gukuramo aside nucleic, GENE DIAN EB-1000 igikoresho cyo gukuramo aside nucleic.
Icyemezo cyibikoresho byubuvuzi Icyemezo No.HJXB No 20210100.
Itariki yo kwemezwa no gusubiramo amabwiriza:Itariki yo kwemezwa: 18 Ugushyingo 2021
Ibyerekeye Twebwe
Nkumushinga wubuhanga buhanitse washinzwe mumwaka wa 2018 ninzobere zikomeye za epigenetike, Epiprobe yibanze ku gusuzuma molekuline ya kanseri ya methylation ya ADN ninganda zuzuye za theranostics.Hamwe nikoranabuhanga ryimbitse, tugamije kuyobora ibihe byibicuruzwa bishya kuri kanseri yonsa!
Dushingiye ku itsinda rya Epiprobe ryibanze ryubushakashatsi bwigihe kirekire, iterambere no guhinduka mubijyanye na methylation ya ADN hamwe nudushya tugezweho, duhujwe na ADN idasanzwe ya methylation yibasiwe na kanseri, dukoresha algorithm idasanzwe itandukanye ihuza amakuru manini nubuhanga bwubwenge bwa artile kugeza wigenga utezimbere tekinoroji yihariye irinzwe na biopsy tekinoroji.Iyo usesenguye urwego rwa methylation rwibibanza byihariye bya ADN yubusa muri sample, hirindwa ibitagenda neza muburyo bwo kwisuzumisha hamwe n’imbogamizi zo kubaga no gutobora ingero, ntibishobora gusa kumenya neza kanseri hakiri kare, ahubwo binatuma hakurikiranwa igihe nyacyo ya kanseri igaragara hamwe niterambere ryiterambere.
