TAGMe ADN Methylation Kumenyekanisha Ibikoresho (qPCR) kuri Kanseri y'inkondo y'umura / Kanseri ya Endometrale
IBIKURIKIRA
Kudatera
Bikurikizwa hamwe na brush cervical brush na Pap smear sample.
Byoroshye
Tekinoroji yambere ya Me-qPCR methylation detection irashobora kurangizwa muntambwe imwe mugihe cyamasaha 3 nta bisulfite ihinduka.
Kera
Kugaragara kurwego rwibanze.
Kwikora
Uherekejwe na software yihariye yo gusesengura ibisubizo, ibisobanuro byibisubizo byikora kandi birasomeka neza.
Ibisabwa
Kwerekana hakiri kare
Abantu bafite ubuzima bwiza
Isuzuma rya Kanseri
Umubare w’abaturage bafite ibyago byinshi (byiza kuri papillomavirus y’abantu bafite ibyago byinshi (hrHPV) cyangwa ibyiza kuri cervical exfoliation cytology / nziza kuri papillomavirus y’abantu bafite ibyago byinshi (hrHPV) cyangwa byiza kuri cytologiya yinkondo y'umura)
Gukurikirana
Umubare w'abaturage
UKORESHEJWE
Iki gikoresho gikoreshwa muri vitro yujuje ubuziranenge bwa hypermethylation ya gene PCDHGB7 muburyo bwa nyababyeyi.Kuri kanseri y'inkondo y'umura, igisubizo cyiza cyerekana ibyago byiyongera byicyiciro cya 2 cyangwa urwego rwo hejuru / rwinshi rwambere rwimbere rwinkondo y'umura (CIN2 +, harimo CIN2, CIN3, adenocarcinoma mumwanya, na kanseri yinkondo y'umura), bisaba ko hasuzumwa colposcopi na / cyangwa histopathologique .Ibinyuranye, ibisubizo bibi byikizamini byerekana ko ibyago bya CIN2 + ari bike, ariko ibyago ntibishobora kuvaho rwose.Isuzuma rya nyuma rigomba gushingira kuri colposcopi na / cyangwa ibisubizo bya histopathologique.Byongeye kandi, kuri kanseri ya endometrale, igisubizo cyiza cyerekana ibyago byinshi byo kwandura endometrale preanserous lesions na kanseri, bisaba ko hasuzumwa histopathologique ya endometrium.Ibinyuranye na byo, ibisubizo bibi by'ibizamini byerekana ko ibyago byo kwandura indwara ya endometrale na kanseri ari bike, ariko ibyago ntibishobora kuvaho rwose.Isuzuma ryanyuma rigomba gushingira kubisubizo bya histopathologique ibisubizo bya endometrium.
PCDHGB7 ni umwe mu bagize umuryango wa protocadherin γ gene cluster.Habonetse Protocadherin igenga imikorere y’ibinyabuzima nko gukwirakwiza ingirabuzimafatizo, ingirabuzimafatizo, apoptose, gutera, kwimuka na autofagy ya selile yibibyimba binyuze mu nzira zitandukanye zerekana ibimenyetso, kandi gucecekesha gene kwayo guterwa na hypermethylation y'akarere ka porotokoro bifitanye isano rya bugufi no kubaho no gutera imbere ya kanseri nyinshi.Byavuzwe ko hypermethylation ya PCDHGB7 ifitanye isano n’ibibyimba bitandukanye, nka lymphoma itari Hodgkin, kanseri y'ibere, kanseri y'inkondo y'umura, kanseri y'inda na kanseri y'uruhago.
IHame RY'INGENZI
Iki gikoresho kirimo gukuramo aside nucleic reagent na reagent ya PCR.Acide nucleique ikurwa muburyo bwa magnetiki-isaro.Iki gikoresho gishingiye ku ihame ryuburyo bwa PCR bwa fluorescence, ukoresheje methylation yihariye-nyayo-nyayo ya PCR kugirango isesengure ADN yerekana icyitegererezo, kandi icyarimwe igaragaze imbuga za CpG za gen PCDHGB7 hamwe nikimenyetso cyo kugenzura ubuziranenge imbere ibice bya G1 na G2.Urwego rwa methylation ya PCDHGB7 murugero, cyangwa agaciro ka Me, rubarwa ukurikije PCDHGB7 gene methylated ADN amplification ya Ct agaciro na Ct agaciro kerekana.PCDHGB7 gene hypermethylation nziza cyangwa mbi igenwa ukurikije agaciro ka Me.
