TAGMe ADN Methylation Detection Kits (qPCR) ya Kanseri y'inkondo y'umura
IBIKURIKIRA
Icyitonderwa
Byemejwe n’amavuriro arenga 36000 mu bushakashatsi bubiri-buhumye-bushakashatsi, ibicuruzwa bifite umwihariko wa 94.3% hamwe na 96.0%.
Byoroshye
Tekinoroji yambere ya Me-qPCR methylation detection irashobora kurangizwa muntambwe imwe mugihe cyamasaha 3 nta bisulfite ihinduka.
Kera
Kwipimisha kanseri y'inkondo y'umura birashobora gutera imbere kugeza kurwego rwo hejuru rwo gukomeretsa (precancerous lesions).
Kwikora
Uherekejwe na software yihariye yo gusesengura ibisubizo, ibisobanuro byibisubizo byikora kandi birasomeka neza.
UKORESHEJWE
Iki gikoresho gikoreshwa muri vitro yujuje ubuziranenge bwa hypermethylation ya gene PCDHGB7 muburyo bwa nyababyeyi.Igisubizo cyiza cyerekana ibyago byiyongera byicyiciro cya 2 cyangwa urwego rwo hejuru / rwinshi rwambere rwimbere rwinkondo y'umura ya neoplasia (CIN2 +, harimo CIN2, CIN3, adenocarcinoma mumwanya, na kanseri y'inkondo y'umura), bisaba ko hakorwa ubundi bushakashatsi bwa colposcopi na / cyangwa histopathologique.Ibinyuranye, ibisubizo bibi byikizamini byerekana ko ibyago bya CIN2 + ari bike, ariko ibyago ntibishobora kuvaho rwose.Isuzuma rya nyuma rigomba gushingira kuri colposcopi na / cyangwa ibisubizo bya histopathologique.PCDHGB7 ni umwe mu bagize umuryango wa protocadherin γ gene cluster.Habonetse Protocadherin igenga imikorere y’ibinyabuzima nko gukwirakwiza ingirabuzimafatizo, ingirabuzimafatizo, apoptose, gutera, kwimuka na autofagy ya selile yibibyimba binyuze mu nzira zitandukanye zerekana ibimenyetso, kandi gucecekesha gene kwayo guterwa na hypermethylation y'akarere ka porotokoro bifitanye isano rya bugufi no kubaho no gutera imbere ya kanseri nyinshi.Byavuzwe ko hypermethylation ya PCDHGB7 ifitanye isano n’ibibyimba bitandukanye, nka lymphoma itari Hodgkin, kanseri y'ibere, kanseri y'inkondo y'umura, kanseri y'inda na kanseri y'uruhago.
IHame RY'INGENZI
Iki gikoresho kirimo gukuramo aside nucleic reagent na reagent ya PCR.Acide nucleique ikurwa muburyo bwa magnetiki-isaro.Iki gikoresho gishingiye ku ihame ryuburyo bwa PCR bwa fluorescence, ukoresheje methylation yihariye-nyayo-nyayo ya PCR kugirango isesengure ADN yerekana icyitegererezo, kandi icyarimwe igaragaze imbuga za CpG za gen PCDHGB7 hamwe nikimenyetso cyo kugenzura ubuziranenge imbere ibice bya G1 na G2.Urwego rwa methylation ya PCDHGB7 murugero, cyangwa agaciro ka Me, rubarwa ukurikije PCDHGB7 gene methylated ADN amplification ya Ct agaciro na Ct agaciro kerekana.PCDHGB7 gene hypermethylation nziza cyangwa mbi igenwa ukurikije agaciro ka Me.
Ibisabwa
Kwerekana hakiri kare
Abantu bafite ubuzima bwiza
Isuzuma rya Kanseri
Abaturage bafite ibyago byinshi (nibyiza kuri papillomavirus yumuntu (hrHPV) cyangwa nibyiza kuri cervical exfoliation cytology)
Gukurikirana
Abaturage nyuma yo kubagwa (hamwe n'amateka yo mu rwego rwo hejuru ibikomere by'inkondo y'umura cyangwa kanseri y'inkondo y'umura)
Ubusobanuro bwa Clinical
Kwipimisha hakiri kare kubaturage bazima:Kanseri y'inkondo y'umura hamwe n'ibisebe bibanziriza bishobora gusuzumwa neza
Isuzuma ry’ingaruka ku baturage bafite ibyago byinshi:Ibyiciro bishobora guterwa muri HPV-nziza yabaturage kugirango bayobore ibyiciro nyuma
Gukurikirana inshuro nyinshi kubaturage nyuma yibikorwa:Igenzura ry’abaturage nyuma yo kubagwa rirashobora gukorwa kugirango hirindwe gutinda kwatewe no kugaruka
Icyegeranyo cy'icyitegererezo
Uburyo bwo gutoranya: Shira igitereko cyimyanya ndangagitsina kuri os cervical os, kwoza buhoro buhoro umwanda winkondo y'umura hanyuma uzenguruke inshuro 4-5 isaha yisaha, ukureho buhoro buhoro umwanda winkondo y'umura, ubishyire mubisubizo byo kubika selile, hanyuma ubishyireho ikimenyetso kugirango ukore ibizamini bikurikira.
Kubungabunga ingero:Ingero zirashobora kubikwa mubushyuhe bwicyumba mugihe cyiminsi 14, kuri 2-8 ℃ mugihe cyamezi 2, na -20 ± 5 ℃ mugihe cyamezi 24.
Igikorwa cyo gutahura: Amasaha 3 (Hatabayeho inzira y'intoki)
Ibikoresho bya ADN Methylation (QPCR) kuri Kanseri ya Urothelia
| Gusaba ivuriro | Kwipimisha kwa Clinical auxiliary ya kanseri y'inkondo y'umura |
| Gene | PCDHGB7 |
| Ubwoko bw'icyitegererezo | Inkondo y'umura y'abagore |
| Uburyo bwo kugerageza | Fluorescence ingano ya tekinoroji ya PCR |
| Icyitegererezo | ABI7500 |
| Gupakira ibisobanuro | Ibizamini 48 |
| Ububiko | Kit A igomba kubikwa kuri 2-30 ℃ Kit B igomba kubikwa kuri -20 ± 5 ℃ Byemewe kugeza kumezi 12 |
